pak pbd beads Search Results


mc2 lhcr lhcf cytochrome psii cp ribosomal proteins cp transcription translation c5 epimerase heat shock protein 70 algae  (Cytoskeleton Inc)
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Cytoskeleton Inc mc2 lhcr lhcf cytochrome psii cp ribosomal proteins cp transcription translation c5 epimerase heat shock protein 70 algae
Mc2 Lhcr Lhcf Cytochrome Psii Cp Ribosomal Proteins Cp Transcription Translation C5 Epimerase Heat Shock Protein 70 Algae, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mc2 lhcr lhcf cytochrome psii cp ribosomal proteins cp transcription translation c5 epimerase heat shock protein 70 algae/product/Cytoskeleton Inc
Average 95 stars, based on 1 article reviews
mc2 lhcr lhcf cytochrome psii cp ribosomal proteins cp transcription translation c5 epimerase heat shock protein 70 algae - by Bioz Stars, 2026-04
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pak pbd agarose beads  (Cytoskeleton Inc)
93
Cytoskeleton Inc pak pbd agarose beads
Inhibition of Rac1 activity through Rac1-LRRK2 interaction in microglia. (A) Rac1 activation assay. Wild-type (WT), control (Cont.), and the two LRRK2-knockdown clones (KD#1 and KD#2) of BV2 microglia cells. <t>PAK-PBD</t> binding to Rac1 and LRRK2 is shown in the upper two panels (PAK-DBD). Total Rac1 and LRRK2 levels are shown in the bottom (total). (B) Relative GTP-Rac1 levels compared to those of total Rac1. N = 3. Values are mean with each data point shown as a dot. (*, P < 0.05 compared to wild-type). Data are representative of ≥3 independent experiments.
Pak Pbd Agarose Beads, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pak pbd agarose beads/product/Cytoskeleton Inc
Average 93 stars, based on 1 article reviews
pak pbd agarose beads - by Bioz Stars, 2026-04
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pak-pbd agarose beads  (Cell Biolabs Inc)
90
Cell Biolabs Inc pak-pbd agarose beads
EGF- but not PDGF-activated Rac1 is inhibited by RV; cdc42 remains unaffected. VSMC were pretreated with RV or vehicle control (DMSO) for 30 min and then stimulated with 20 ng/mL EGF (A, C) or 2 ng/mL PDGF (B, C) as indicated for 5 min (for Rac1) and 2 min (for cdc42). Cells were subsequently lysed, and an aliquot was used to determine total Rac1 or total cdc42. The remaining lysate was incubated with <t>PAK-PBD</t> agarose beads for 45 min. Active pulled-down Rac1 was correlated to total Rac1 levels. Bar graphs show the mean±SEM of four independent experiments ( * p <0.05; ** p <0.01; ns, not significant). Representative Western Blots are shown.
Pak Pbd Agarose Beads, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pak-pbd agarose beads/product/Cell Biolabs Inc
Average 90 stars, based on 1 article reviews
pak-pbd agarose beads - by Bioz Stars, 2026-04
90/100 stars
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pak-1 pbd agarose beads  (Merck KGaA)
90
Merck KGaA pak-1 pbd agarose beads
EGF- but not PDGF-activated Rac1 is inhibited by RV; cdc42 remains unaffected. VSMC were pretreated with RV or vehicle control (DMSO) for 30 min and then stimulated with 20 ng/mL EGF (A, C) or 2 ng/mL PDGF (B, C) as indicated for 5 min (for Rac1) and 2 min (for cdc42). Cells were subsequently lysed, and an aliquot was used to determine total Rac1 or total cdc42. The remaining lysate was incubated with <t>PAK-PBD</t> agarose beads for 45 min. Active pulled-down Rac1 was correlated to total Rac1 levels. Bar graphs show the mean±SEM of four independent experiments ( * p <0.05; ** p <0.01; ns, not significant). Representative Western Blots are shown.
Pak 1 Pbd Agarose Beads, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pak-1 pbd agarose beads/product/Merck KGaA
Average 90 stars, based on 1 article reviews
pak-1 pbd agarose beads - by Bioz Stars, 2026-04
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p21-binding domain (pbd) pak fused glutathione s-transferase (gst) agarose beads  (Cell Biolabs Inc)
90
Cell Biolabs Inc p21-binding domain (pbd) pak fused glutathione s-transferase (gst) agarose beads
EGF- but not PDGF-activated Rac1 is inhibited by RV; cdc42 remains unaffected. VSMC were pretreated with RV or vehicle control (DMSO) for 30 min and then stimulated with 20 ng/mL EGF (A, C) or 2 ng/mL PDGF (B, C) as indicated for 5 min (for Rac1) and 2 min (for cdc42). Cells were subsequently lysed, and an aliquot was used to determine total Rac1 or total cdc42. The remaining lysate was incubated with <t>PAK-PBD</t> agarose beads for 45 min. Active pulled-down Rac1 was correlated to total Rac1 levels. Bar graphs show the mean±SEM of four independent experiments ( * p <0.05; ** p <0.01; ns, not significant). Representative Western Blots are shown.
P21 Binding Domain (Pbd) Pak Fused Glutathione S Transferase (Gst) Agarose Beads, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p21-binding domain (pbd) pak fused glutathione s-transferase (gst) agarose beads/product/Cell Biolabs Inc
Average 90 stars, based on 1 article reviews
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gst-pak-pbd fusion protein conjugated to glutathione beads  (Cell Biolabs Inc)
90
Cell Biolabs Inc gst-pak-pbd fusion protein conjugated to glutathione beads
EGF- but not PDGF-activated Rac1 is inhibited by RV; cdc42 remains unaffected. VSMC were pretreated with RV or vehicle control (DMSO) for 30 min and then stimulated with 20 ng/mL EGF (A, C) or 2 ng/mL PDGF (B, C) as indicated for 5 min (for Rac1) and 2 min (for cdc42). Cells were subsequently lysed, and an aliquot was used to determine total Rac1 or total cdc42. The remaining lysate was incubated with <t>PAK-PBD</t> agarose beads for 45 min. Active pulled-down Rac1 was correlated to total Rac1 levels. Bar graphs show the mean±SEM of four independent experiments ( * p <0.05; ** p <0.01; ns, not significant). Representative Western Blots are shown.
Gst Pak Pbd Fusion Protein Conjugated To Glutathione Beads, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst-pak-pbd fusion protein conjugated to glutathione beads/product/Cell Biolabs Inc
Average 90 stars, based on 1 article reviews
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recombinant proteins prefixed colored glutathione sepharose beads  (Cytoskeleton Inc)
90
Cytoskeleton Inc recombinant proteins prefixed colored glutathione sepharose beads
EGF- but not PDGF-activated Rac1 is inhibited by RV; cdc42 remains unaffected. VSMC were pretreated with RV or vehicle control (DMSO) for 30 min and then stimulated with 20 ng/mL EGF (A, C) or 2 ng/mL PDGF (B, C) as indicated for 5 min (for Rac1) and 2 min (for cdc42). Cells were subsequently lysed, and an aliquot was used to determine total Rac1 or total cdc42. The remaining lysate was incubated with <t>PAK-PBD</t> agarose beads for 45 min. Active pulled-down Rac1 was correlated to total Rac1 levels. Bar graphs show the mean±SEM of four independent experiments ( * p <0.05; ** p <0.01; ns, not significant). Representative Western Blots are shown.
Recombinant Proteins Prefixed Colored Glutathione Sepharose Beads, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant proteins prefixed colored glutathione sepharose beads/product/Cytoskeleton Inc
Average 90 stars, based on 1 article reviews
recombinant proteins prefixed colored glutathione sepharose beads - by Bioz Stars, 2026-04
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gst tagged pak-pbd protein beads  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc gst tagged pak-pbd protein beads
EGF- but not PDGF-activated Rac1 is inhibited by RV; cdc42 remains unaffected. VSMC were pretreated with RV or vehicle control (DMSO) for 30 min and then stimulated with 20 ng/mL EGF (A, C) or 2 ng/mL PDGF (B, C) as indicated for 5 min (for Rac1) and 2 min (for cdc42). Cells were subsequently lysed, and an aliquot was used to determine total Rac1 or total cdc42. The remaining lysate was incubated with <t>PAK-PBD</t> agarose beads for 45 min. Active pulled-down Rac1 was correlated to total Rac1 levels. Bar graphs show the mean±SEM of four independent experiments ( * p <0.05; ** p <0.01; ns, not significant). Representative Western Blots are shown.
Gst Tagged Pak Pbd Protein Beads, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gst tagged pak-pbd protein beads/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
gst tagged pak-pbd protein beads - by Bioz Stars, 2026-04
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Image Search Results


Inhibition of Rac1 activity through Rac1-LRRK2 interaction in microglia. (A) Rac1 activation assay. Wild-type (WT), control (Cont.), and the two LRRK2-knockdown clones (KD#1 and KD#2) of BV2 microglia cells. PAK-PBD binding to Rac1 and LRRK2 is shown in the upper two panels (PAK-DBD). Total Rac1 and LRRK2 levels are shown in the bottom (total). (B) Relative GTP-Rac1 levels compared to those of total Rac1. N = 3. Values are mean with each data point shown as a dot. (*, P < 0.05 compared to wild-type). Data are representative of ≥3 independent experiments.

Journal: Animal Cells and Systems

Article Title: LRRK2 decreases microglial actin dynamics by filamentous actin depolymerization and Rac1 inhibition

doi: 10.1080/19768354.2022.2158219

Figure Lengend Snippet: Inhibition of Rac1 activity through Rac1-LRRK2 interaction in microglia. (A) Rac1 activation assay. Wild-type (WT), control (Cont.), and the two LRRK2-knockdown clones (KD#1 and KD#2) of BV2 microglia cells. PAK-PBD binding to Rac1 and LRRK2 is shown in the upper two panels (PAK-DBD). Total Rac1 and LRRK2 levels are shown in the bottom (total). (B) Relative GTP-Rac1 levels compared to those of total Rac1. N = 3. Values are mean with each data point shown as a dot. (*, P < 0.05 compared to wild-type). Data are representative of ≥3 independent experiments.

Article Snippet: Cleared lysates were incubated with PAK-PBD agarose beads (Cytoskeleton, USA) for 1 h at 4°C.

Techniques: Inhibition, Activity Assay, Activation Assay, Clone Assay, Binding Assay

EGF- but not PDGF-activated Rac1 is inhibited by RV; cdc42 remains unaffected. VSMC were pretreated with RV or vehicle control (DMSO) for 30 min and then stimulated with 20 ng/mL EGF (A, C) or 2 ng/mL PDGF (B, C) as indicated for 5 min (for Rac1) and 2 min (for cdc42). Cells were subsequently lysed, and an aliquot was used to determine total Rac1 or total cdc42. The remaining lysate was incubated with PAK-PBD agarose beads for 45 min. Active pulled-down Rac1 was correlated to total Rac1 levels. Bar graphs show the mean±SEM of four independent experiments ( * p <0.05; ** p <0.01; ns, not significant). Representative Western Blots are shown.

Journal: Molecular Nutrition & Food Research

Article Title: Resveratrol inhibits migration and Rac1 activation in EGF- but not PDGF-activated vascular smooth muscle cells

doi: 10.1002/mnfr.201100309

Figure Lengend Snippet: EGF- but not PDGF-activated Rac1 is inhibited by RV; cdc42 remains unaffected. VSMC were pretreated with RV or vehicle control (DMSO) for 30 min and then stimulated with 20 ng/mL EGF (A, C) or 2 ng/mL PDGF (B, C) as indicated for 5 min (for Rac1) and 2 min (for cdc42). Cells were subsequently lysed, and an aliquot was used to determine total Rac1 or total cdc42. The remaining lysate was incubated with PAK-PBD agarose beads for 45 min. Active pulled-down Rac1 was correlated to total Rac1 levels. Bar graphs show the mean±SEM of four independent experiments ( * p <0.05; ** p <0.01; ns, not significant). Representative Western Blots are shown.

Article Snippet: Rac1 and cdc42 activation assay kits including PAK-PBD agarose beads and Western Blot antibodies targeting Rac1 and cdc42 were bought from Cell Biolabs (San Diego, CA, USA).

Techniques: Control, Incubation, Western Blot